Dr. Miriam Filippi

Lecturer at the Department of Mechanical and Process Engineering

ETH Zürich

Professur für Robotik

CLA F 7

Tannenstrasse 3

8092 Zürich

Switzerland

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Additional information

Research area

Soft robotics can be greatly advanced by unique features found in living systems (self-healing, softness, biocompatibility, energy conversion efficiency, degradability).

My aim is to find manufacturing and biological strategies to make living systems become effective engineering materials. Engineered muscle tissue is an extraordinary multifunctional platform that is capable of controllable deformation (ie, bio-actuation), and serves for robotic and biomedical applications. My work focuses on generating 3D muscle tissue-constructs, integrating them with artificial elements and technologies, and using them as biomodules for actuation, sensing, and signal communication in bio-hybrid robots.

Key Active Projects:

High-performance, durable engineered muscle tissue. This project focuses on achieving higher force output and improving actuation performance of 3D bio-actuator designs by increasing the size of engineered skeletal muscle tissue and enabling its full maturity. We look into high-resolution biofabrication and microfluidics to understand how to realize intratissular perfusable architectures for sufficient nutrient and gaseous exchange for the cells to survive, and enable the scaling up of engineered tissue beyond the cm-scale. https://www.youtube.com/watch?v=P7THO95V6Locall_made
Autonomous systems for bio-actuators. This project focuses on integrating novel functions into bio-actuators that enable automaticity in their operation. To integrate novel functions in our engineered muscles, we look into responsive, functional materials that can be prepared as soft formulations and seamlessly integrated with cell-loading hydrogels that forms the engineered muscle in vitro.
Integrative designs for neuromuscular tissue models. This project looks at combining biological actuators cells (skeletal muscle) with their natural controllers (motoneurons) and studing the bio-electronics behind signal transmission in 3D models. Here, we aim to solve design problems (e.g., fine distribution of neural cells and cm-scaled designs), and understand the applicability to biomedical applications of system realized from medically relevant cells (e.g., iPSCs-derived motoneurons).
Sustainability in biofabrication. This project aims at reducing resource and time that are needed to fabricate engineered muscle tissue models. We look into computed modeling approaches to select optimized muscle designs and raw materials that match defined bio-actuation metrics, thus allowing us to refine the process of biofabrication.

Profile: Bioengineer with a neurobiological background and expertise in Tissue Engineering, Regenerative Medicine, Molecular Imaging, Bio-hybrid Systems, and Nanotechnology.

Previous and current research areas: regeneration and tissue engineering in skeletal/cardiac muscle tissue, neuromuscular tissue, spinal cord, nerve, bone, and cartilage tissue.

Focus: engineering of complex tissue models (e.g., biomimetic architectures, vascularization/perfusion strategies, heterocellular cultures, multi-tissue models, and bio-hybrid interfaces); biophysical principles (e.g., magnetic actuation; nanoparticle-mediated photoreactivity, magneto-sonoporation, optogenetics) for remote modulation of cell functions (e.g., stem cell differentiation, muscle cell contraction, membrane depolarization); molecular imaging probes for cancer and injury detection (e.g., hypoxia-sensitive probes, theranostic approaches); cell therpay and in vivo surveillance of transplanted regenerative cells.

Professional experience

2022-current: Senior Researcher in Growing, Fabricating and Controlling of Biohybrid Robot. Soft Robotics Laboratory, ETH Zurich, Switzerland.

Lecturer (Master Courses: "Distinguished Lecture Series on Engineering with Living Materials", MaP ETH; "Cells and Technologies in Regenerative Surgery" UniBasel)

Biosafety Officer (Level I and II), Biolab Manager, Sustainibility Officer.

Scientific coordinator of: "3D Bioprinting of Muscle-Tendon Units" (ETHZ - IBEC); "Bioxolography and machine learning to engineer skeletal muscle tissue for actuation" (ETHZ - UniBasel - EOC - Humboldt Uni); "Biofabrication of Neuromuscular Tissue Models" (ETHZ); "Multiscale Biohybrid Tissues for Robotics Driven by Applications in Health" (ETHZ - ALIVE); "Hydrogel-based Strategies for Selective Actuation Control in Engineered Skeletal Muscle" (ETHZ - EPFL); "Sensorized Bio-actuators for Autonomous Bio-robotics" (ETHZ - EMPA); "Perfusion Strategies for Scalable Engineered Tissue" (ETHZ).

2021-2022: Post-Doc Researcher in Growing, Fabricating and Controlling of Biohybrid Robot. Soft Robotics Laboratory, ETH Zurich, Switzerland.

2017-2020: Post-Doc Researcher in Nanomaterials for Tissue Engineering and Regenerative Medicine. Laboratory of Tissue Engineering, University Hospital of Basel, Switzerland.

2013-2016: PhD in Pharmaceutical and Biomolecular Sciences. Molecular Imaging Centre, University of Turin, Italy.

2014-2015: Teaching assistant in General and Inorganic Chemistry. Faculty of Pharmacy, University of Turin, Italy.

2012: Scientific Associate in Molecular Imaging. Molecular Imaging Centre, University of Turin, Italy.

2010-2012: M.Sc. in Molecular Biotechnology and Imaging, University of Turin, Italy.

2011-2012: Teaching assistant in Molecular Tumor Immunology. Laboratory of Tumor Immunology, University of Turin, Italy.

2010-2012: Scientific trainee in Molecular Tumor Immunology. Laboratory of Tumor Immunology, University of Turin, Italy.

2006-2010: B.Sc. in Molecular Biotechnology, University of Turin, Italy.

Main publications

Filippi M, et al. Biohybrid nanointerfaces for neuromodulation. Nano Today. 2024. Doi: 10.1016/j.nantod.2023.102094
Filippi M, et al. Perfusable biohybrid designs for bioprinted skeletal muscle tissue. Adv Health Mat. 2023. Doi: 10.1002/adhm.202300151
Filippi M, et al. Will microfluidics enable functionally integrated biohybrid robots?, PNAS. 2022. Doi: 10.1073/pnas.2200741119
Garello F, .., and Filippi M. Micro/Nanosystems for Magnetic Targeted Delivery of Bioagents Biomedicine. 2022. Doi: 10.3390/pharmaceutics14061132
Filippi M, et al. Microfluidic Tissue Engineering and Bio-Actuation. Adv Mat. 2022. Doi:10.1002/adma.202108427.
Filippi M, et al. Engineered Magnetic Nanocomposites to Modulate Cellular Function. Small. 2022. Doi: 10.1002/smll.202104079.
Filippi M, et al. Strategies to promote vascularization, survival, and functionality of engineered tissues. (Chapter 13), Tissue Engineering 3rd Edition, 14, 2021.
Bitonto V, ..., and Filippi M. Prussian blue staining to visualize iron oxide nanoparticles. Histochemistry of Single Molecules. Met Mol Biol, Springer, 2021. Doi: 10.1007/978-1-0716-2675-7_26
Filippi M, et al. Rapid Magneto-Sonoporation of Adipose-Derived Cells. Materials. 2021. Doi: 10.3390/ma14174877.
Jalili-Firoozinezhad S,* Filippi M,* et al. Chicken egg white: hatching of a new old biomaterial. Mat Today. 2020. Doi: 10.1016/j.mattod.2020.05.022. *First authors.
Filippi M, et al. Natural polymeric scaffolds in bone regeneration. Front Bioeng Biotechnol. 2020, 8, 474. Doi: 10.3389/fbioe.2020.00474.
Filippi M, et al. Metronidazole-functionalized iron oxide nanoparticles for molecular targeting of hypoxic tissue. Nanoscale. 2019,11, 22559-74. Doi: 10.1039/C9NR08436C.
Filippi M, et al. Use of nanoparticles in skeletal tissue regeneration and engineering. Histol Histopathol. 2019, 18184. Doi: 10.14670/HH-18-184.
Filippi M, et al. Indocyanine Green for optical and photoacoustic imaging of Mesenchymal Stem Cells after in vivo transplantation. J Biophotonics. 2019, 12(5):e201800035. Doi: 10.1002/jbio.201800035.
Filippi M, et al. Magnetic nanocomposite hydrogels and static magnetic field stimulate the osteoblastic and vasculogenic profile of adipose-derived cells. Biomaterials. 2019, 223, 119468. Doi: 10.1016/j.biomaterials.2019.119468.
Filippi M, et al. Imaging of MSC transplantation in neuroscience. Oncotarget. 2017, 8, 10781-82. Doi: 10.18632/oncotarget.14643.
Filippi M, et al. First in vivo MRI study on theranostic dendrimersomes. J Control Release. 2017, 248, 45-52. Doi: 10.1016/j.jconrel.2017.01.010.
Filippi M, et al. Successful in vivo MRI tracking of MSCs labelled with Gadoteridol in a Spinal Cord Injury experimental model. Exp Neurol. 2016, 282, 66-77. Doi: 10.1016/j.expneurol.2016.05.023.
Filippi M, et al. GdDOTAGA(C₁₈)₂: an efficient amphiphilic Gd(III) chelate for the preparation of self-assembled high relaxivity MRI nanoprobes. Chem Commun (Camb). 2015, 51, 17455-8. Doi: 10.1039/c5cc06032j.
Filippi M, et al. Novel stable dendrimersome formulation for safe bioimaging applications. Nanoscale. 2015, 7, 12943-54. Doi: 10.1039/c5nr02695d.
Filippi M, et al. Dendrimersomes: a new vesicular nano-platform for MR-molecular imaging applications. Chem Commun (Camb). 2014, 50, 3453-6. Doi: 10.1039/c3cc49584a.

Other Publications

Yasa O, et al. Perforated red blood cells enable compressible and injectable hydrogels as therapeutic vehicles. Materials Today 2023, 72, 36. Doi: 10.1016/j.mattod.2023.11.004.
Garello F, et al. Imaging of Inflammation in Spinal Cord Injury: Novel Insights on the Usage of PFC-Based Contrast Agents. Biomedicines 2021, 9, 379.
Nguyen DV, et al. Mastering bioactive coatings of metal oxide nanoparticles and surfaces through phosphonate dendrons. New J Chem, 2020, 44, 3206-14. Doi: 10.1039/C9NJ05565G.
Pigeot S, et al. Manufacturing of human tissues as off-the-shelf grafts programmed to induce regeneration. Adv Mater, 2021, \e2103737. Doi: 10.1002/adma.202103737
Filippi M, et al. Magnetic nanocomposite hydrogels combined with static magnetic field for enhanced bone regeneration. eCM Meeting Abstracts. 2018, 2, 9.
Siemer A et al., Nano meets micro-translational nanotechnology in medicine: nano-based applications for early tumor detection and therapy. Nanomaterials (Basel). 2020, 10. Doi: 10.3390/nano10020383.
Pallavicini G, et al. Inactivation of citron kinase inhibits medulloblastoma progression by inducing apoptosis and cell Senescence. Cancer Res. 2018, 78, 4599-4612. Doi: 10.1158/0008-5472.CAN-17-4060.
Garello F, et al. Glucan particles loaded with a NIRF agent for imaging monocytes/macrophages recruitment in a model of rheumatoid arthritis. RSC Adv. 2015, 5, 34078-87. Doi: 10.1039/C5RA00720H.
Giustetto P, et al. Non-invasive parenchymal, vascular and metabolic high-frequency ultrasound and photoacoustic rat deep brain imaging. J Vis Exp. 2015, 97. Doi: 10.3791/52162
Pierobon D, et al. Chronic hypoxia reprograms human immature dendritic cells by inducing a proinflammatory phenotype and TREM-1 expression. Eur J Immunol. 2013, 43, 949-66. Doi: 10.1002/eji.201242709.